Pfu Mastermix is a ready-to-use 2X solution of pfu DNA polymerase, dNTPs, MgCl₂, and extreme performance for all major PCR applications. Incorporating an exciting new technology, pfu DNA Polymerase brings together a novel Escherichia-like enzyme with a processivity-enhancing domain. The pfu DNA Polymerase generates long templates with accuracy and speed previously unattainable with a single enzyme, even on the most difficult templates. pfu DNA Polymerase possesses the following activities: 5´→3´ DNA polymerase activity and 3´→5´ exonuclease activity. It generates blunt ends in the amplification products. Pfu Mastermix is a convenient 2X mix containing pfu DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl₂. Only template and primers need to be added by the user.