2X Taq Mastermix is a ready-to-use 2X solution of Taq DNA polymerase, dNTPs, MgCl₂, and reaction buffers at optimal concentrations for efficient PCR amplification of DNA templates in the range of 0.2–2kb. The mastermix is convenient to use and reduces the chances of contamination by reducing the number of pipetting steps. It helps in quickly setting up the reaction in a minute or two and also in optimizing PCR conditions by changing only either template or primer concentration without adding individual components. The master mix is stable for twelve months. The mix is optimized for efficient amplification of all the templates and results in reproducible PCR.